LOCUS       40924_18986        7260 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BAC cloning vector pEZ BAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Godiska R, Mead DA.
  TITLE     CopyRight pEZ BAC vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Godiska R, Mead DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2008) Lucigen Corp., 2120 W. Greenview Dr. #9, 
            Middleton, WI 53562, USA
REFERENCE   3  (bases 1 to 7260)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-AUG-2008) Lucigen Corp., 2120 W. Greenview Dr. #9, Middleton, WI
            53562, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..57)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_recomb     79..112
                     /label=loxP site #1
                     /note="loxP site #1"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    198..219
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        234..264
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    272..288
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     296..312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    377..382
                     /label=HpaI blunt cloning site
                     /note="HpaI blunt cloning site"
     promoter        complement(411..429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(436..452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(632..665)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(684..715)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     misc_feature    764..1162
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        1185..1287
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1288..1944
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    complement(2218..2691)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2767..3735)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(3738..4910)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(5236..5486)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5492..6244)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(6335..6554)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(6628..7257)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
       61 gagttgattg ctacgtaaat aacttcgtat agcatacatt atacgaagtt atggactagg
      121 cgcgccagaa gagagaaaga aggaaagcgg ccgcacaggt ttcccgactg gaaagcgggc
      181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      301 aacagctatg accatgatta cgccaagcta tttaggtgag actatagaat actcaagctt
      361 gcatgcgata cgtatcgtta acgatggatc cgacgcacgt gcgaattcgc cctatagtga
      421 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      481 cacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      541 ggcccgcacc gatcgccctt cccaacagtt gcgcagctga atggcgaatg gcgcctgatg
      601 cggtattttc tccttacggc ggccgcttga cataacttcg tatagcatac attatacgaa
      661 gttatgttta aacattagca gaaagtcaaa ggcctccggt cggaggcttt tgactaaaac
      721 ttcccttggg gttatcattg ggccgagacc gcctgaagag gacttccatt gttcattcca
      781 cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag ctcgctttca gcacctgtcg
      841 tttcctttct tttcagaggg tattttaaat aaaaacatta agttatgacg aagaagaacg
      901 gaaacgcctt aaaccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg
      961 taacctgtcg gatcaccgga aaggacccgt aaagtgataa tgattatcat ctacatatca
     1021 caacgtgcgt ggaggccatc aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat
     1081 taattgatct gcatcaactt aacgtaaaag caacttcaga caatacaaat cagcgacact
     1141 gaatacgggg caacctcatg tcgcctgaag agtgagaccg gccctgatcg gcacgtaaga
     1201 ggctccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     1261 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     1321 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     1381 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     1441 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1501 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1561 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1621 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1681 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1741 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1801 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1861 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1921 tgcgatgagt ggcagggcgg ggcgtaaaaa tgtaatcacc tggctcacct tcgggtgggc
     1981 ctttcacact tgcatcggat gcagcccggt gaacgtgccg gcacggcctg ggtaaccagg
     2041 tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca gcagcaatcc
     2101 acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg acgacatgtc
     2161 aatacttgcc cttgacaggc attgatggaa tcgtagtctc acgctgatag tctgatcgac
     2221 aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga gtgggatcgt
     2281 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gactaataat
     2341 cagaccgacg atacgagtgg gaccgtggtt ccagactaat aatcagaccg acgatacgag
     2401 tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc atggtcccag
     2461 actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt atcagaccga
     2521 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg
     2581 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta
     2641 tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc cagttatgct
     2701 ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta aaacgtggtc
     2761 gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct ctatacactc
     2821 agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat acaatactgt
     2881 cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag atgacgtttt
     2941 aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc cagctttttt
     3001 ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg taaaggcttt
     3061 ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac
     3121 tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg aaatattttc
     3181 cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc ttgcataacg
     3241 ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg ataatgcagc
     3301 catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg taagtgcagc
     3361 agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc ttcgaccgaa
     3421 cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca gtgcgtcctc
     3481 agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct tctcaacact
     3541 atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag gcaaagtaat
     3601 ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca tcggggcagc
     3661 tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt ttggaataac
     3721 aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc gaaaatttca
     3781 ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga agagcgttga
     3841 tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac ttcatccgtt
     3901 tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg ctcctccatc
     3961 cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg tacatcaggc
     4021 tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa aaactgcagt
     4081 gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc agcagcacat
     4141 acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat gacatcatag
     4201 tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt gggcagttta
     4261 ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag acaggaagga
     4321 ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc gtccttttcc
     4381 ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg tacccatccg
     4441 tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc
     4501 ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac gccaccttta
     4561 tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca
     4621 aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg ctcaacccgt
     4681 cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc tctgatagcc
     4741 tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg ggttattttc
     4801 ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt catttcatga
     4861 ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat cctttaatca
     4921 ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac aaaatcgcaa
     4981 agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa ggagataaga
     5041 agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg taaccgagca
     5101 tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc tcttacgctc
     5161 agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac acgcggatag
     5221 ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga actaaccccc
     5281 gatatcaagt cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca
     5341 aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag
     5401 gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg acgaaccacc
     5461 ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg atatcgcgga
     5521 aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca
     5581 ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag
     5641 gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag
     5701 agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat aaacgcatgg
     5761 catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta
     5821 acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg
     5881 gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca tcgccggcat
     5941 cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta
     6001 tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga
     6061 tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga
     6121 acagatacag catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt
     6181 ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg
     6241 ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga
     6301 gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag
     6361 ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt cacactgaaa
     6421 gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc
     6481 taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag
     6541 tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg
     6601 ttccctggtg cttcctcgct cactcgaccg ggagggttcg agaagggggg gcacccccct
     6661 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     6721 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     6781 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     6841 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     6901 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     6961 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     7021 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     7081 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
     7141 aacgccggcg gccggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
     7201 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
//
